Imaging and Defining Emergent Behaviors of the Immune Response

ZipSeq Resources and Protocols (Note this is still a work in progress, thanks for your patience)

  • ZipSeq Reagents Setup and QC version1: This short guide will take you through the best practices I've found for generating the oligo-Ab conjugates described in our Nat. Methods paper and how to QC them on test cells w/ flow cytometry. It also lays out how to test hybridization of strands and photouncaging 'binariness' 
  • ZipSeq Sectioning version1: This short guide takes you through how to section your tissue well which is critical for the zipseq method. This document is performed on lymph nodes as an example, but works with PyMT tumors as well.
  • Visual Basics Plugin (by John Eichorst) for automated ROI generation across multiple FOV for Mosaic targeted illumination through Metamorph: https://github.com/BIDCatUCSF/VB-Plugin-for-Patterned-Illumination

Lab Data

Lab Tools

Microscopy Related

Building a 2-Photon Microscope

GitHub Entries for Automation and Analysis Routines

Micromagellan for Tissue Exploration

Intravital Lung Imaging Instructions

 

Other Lab Equipment

 

 

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